Cell-free protein synthesis in microfluidic 96-well plates

Kirsten Jackson; Ruba Khnouf; Z Hugh Fan

doi:10.1007/978-1-62703-782-2_10

Cell-free protein synthesis in microfluidic 96-well plates

Methods Mol Biol. 2014:1118:157-68. doi: 10.1007/978-1-62703-782-2_10.

Authors

Kirsten Jackson¹, Ruba Khnouf, Z Hugh Fan

Affiliation

¹ Department of Biomedical Engineering, University of Florida, Gainesville, FL, USA.

PMID: 24395415
DOI: 10.1007/978-1-62703-782-2_10

Abstract

Cell-free protein synthesis (CFPS) enables rapid protein expression for the structural and functional characterization of proteins. Implementation of CFPS in a microfluidic platform has additional benefits such as reduced reaction volumes and simultaneous expression of multiple proteins. Here, we describe a microfluidic device that is composed of 96 continuous-exchange cell-free protein expression units and produces a protein synthesis yield up to 87 times higher than a conventional batch system.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Microfluidic Analytical Techniques / instrumentation
Microfluidic Analytical Techniques / methods*
Protein Biosynthesis*
Templates, Genetic
Time Factors