Telomerase governs immunomodulatory properties of mesenchymal stem cells by regulating FAS ligand expression

EMBO Mol Med. 2014 Mar;6(3):322-34. doi: 10.1002/emmm.201303000. Epub 2014 Jan 8.

Abstract

Bone marrow mesenchymal stem cells (BMMSCs) are capable of differentiating into multiple cell types and regulating immune cell response. However, the mechanisms that govern the immunomodulatory properties of BMMSCs are still not fully elucidated. Here we show that telomerase-deficient BMMSCs lose their capacity to inhibit T cells and ameliorate the disease phenotype in systemic sclerosis mice. Restoration of telomerase activity by telomerase reverse transcriptase (TERT) transfection in TERT(-/-) BMMSCs rescues their immunomodulatory functions. Mechanistically, we reveal that TERT, combined with β-catenin and BRG1, serves as a transcriptional complex, which binds the FAS ligand (FASL) promoter to upregulate FASL expression, leading to an elevated immunomodulatory function. To test the translational value of these findings in the context of potential clinical therapy, we used aspirin treatment to upregulate telomerase activity in BMMSCs, and found a significant improvement in the immunomodulatory capacity of BMMSCs. Taken together, these findings identify a previously unrecognized role of TERT in improving the immunomodulatory capacity of BMMSCs, suggesting that aspirin treatment is a practical approach to significantly reduce cell dosage in BMMSC-based immunotherapies.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipogenesis
  • Animals
  • Anti-Inflammatory Agents, Non-Steroidal / pharmacology
  • Aspirin / pharmacology
  • Bone Marrow Cells / cytology
  • Cell Differentiation
  • Cells, Cultured
  • DNA Helicases / metabolism
  • Disease Models, Animal
  • Fas Ligand Protein / genetics
  • Fas Ligand Protein / metabolism*
  • Gene Expression Regulation*
  • Mesenchymal Stem Cell Transplantation
  • Mesenchymal Stem Cells / cytology
  • Mesenchymal Stem Cells / drug effects
  • Mesenchymal Stem Cells / metabolism*
  • Mice
  • Mice, Inbred C57BL
  • Nuclear Proteins / metabolism
  • Promoter Regions, Genetic
  • Scleroderma, Systemic / metabolism
  • Scleroderma, Systemic / pathology
  • Scleroderma, Systemic / surgery
  • T-Lymphocytes / immunology
  • T-Lymphocytes / metabolism
  • Telomerase / deficiency
  • Telomerase / genetics
  • Telomerase / metabolism*
  • Transcription Factors / metabolism
  • beta Catenin / antagonists & inhibitors
  • beta Catenin / metabolism

Substances

  • Anti-Inflammatory Agents, Non-Steroidal
  • Fas Ligand Protein
  • Nuclear Proteins
  • Transcription Factors
  • beta Catenin
  • Telomerase
  • Smarca4 protein, mouse
  • DNA Helicases
  • Aspirin