Abstract
An efficient one-pot three enzymes strategy for chemoenzymatic synthesis of ADP-d-glycero-β-d-manno-heptose (ADP-d, d-heptose) was reported using chemically synthesized d, d-heptose-7-phosphate and the ADP-d, d-heptose biosynthetic enzymes HldE and GmhB. Moreover, the result of investigating substrate specificity of the kinase action of HldE revealed that HldE had highly restricted substrate specificity towards structurally modified heptose-7-phosphate analogs.
Keywords:
Analogs; Chemoenzymatic; Specificity; Synthesis.
Published by Elsevier Ltd.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenosine Diphosphate Sugars / chemical synthesis*
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Adenosine Diphosphate Sugars / metabolism
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Chemistry Techniques, Synthetic
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Multienzyme Complexes / metabolism*
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Nucleotidyltransferases / metabolism*
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Phosphotransferases (Alcohol Group Acceptor) / metabolism*
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Substrate Specificity
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Sugar Phosphates / chemistry
Substances
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Adenosine Diphosphate Sugars
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Multienzyme Complexes
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Sugar Phosphates
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Phosphotransferases (Alcohol Group Acceptor)
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Nucleotidyltransferases
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D,D heptose 7-phosphate kinase-D,D-heptose 1-phosphate adenylyltransferase, E coli