Abstract
Cellular HIV-1 reservoirs that persist despite antiretroviral treatment are incompletely defined. We show that during suppressive antiretroviral therapy, CD4(+) T memory stem cells (TSCM cells) harbor high per-cell levels of HIV-1 DNA and make increasing contributions to the total viral CD4(+) T cell reservoir over time. Moreover, we conducted phylogenetic studies that suggested long-term persistence of viral quasispecies in CD4(+) TSCM cells. Thus, HIV-1 may exploit the stem cell characteristics of cellular immune memory to promote long-term viral persistence.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Antiretroviral Therapy, Highly Active
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Base Sequence
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Boston
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CD4-Positive T-Lymphocytes / cytology
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CD4-Positive T-Lymphocytes / virology*
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Cluster Analysis
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Disease Reservoirs / virology*
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Evolution, Molecular
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Flow Cytometry
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HIV Infections / drug therapy
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HIV Infections / virology*
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HIV-1 / genetics*
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Humans
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Lymphoid Progenitor Cells / cytology*
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Molecular Sequence Data
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Phylogeny*
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Reverse Transcriptase Polymerase Chain Reaction
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Sequence Analysis, DNA
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Species Specificity
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Statistics, Nonparametric
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Virus Latency*
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env Gene Products, Human Immunodeficiency Virus / genetics
Substances
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env Gene Products, Human Immunodeficiency Virus