Arboretum and Puerto Almendras viruses: two novel rhabdoviruses isolated from mosquitoes in Peru

Nikos Vasilakis; Fanny Castro-Llanos; Steven G Widen; Patricia V Aguilar; Hilda Guzman; Carolina Guevara; Roberto Fernandez; Albert J Auguste; Thomas G Wood; Vsevolod Popov; Kirk Mundal; Elodie Ghedin; Tadeusz J Kochel; Edward C Holmes; Peter J Walker; Robert B Tesh

doi:10.1099/vir.0.058685-0

Arboretum and Puerto Almendras viruses: two novel rhabdoviruses isolated from mosquitoes in Peru

J Gen Virol. 2014 Apr;95(Pt 4):787-792. doi: 10.1099/vir.0.058685-0. Epub 2014 Jan 13.

Authors

Nikos Vasilakis^{1

2

3}, Fanny Castro-Llanos⁴, Steven G Widen⁵, Patricia V Aguilar^{1

2

3}, Hilda Guzman³, Carolina Guevara⁴, Roberto Fernandez⁴, Albert J Auguste^{1

3}, Thomas G Wood⁵, Vsevolod Popov^{1

3}, Kirk Mundal⁴, Elodie Ghedin⁶, Tadeusz J Kochel⁷, Edward C Holmes⁸, Peter J Walker⁹, Robert B Tesh^{1

2

3}

Affiliations

¹ Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, TX, USA.
² Center for Tropical Diseases, University of Texas Medical Branch, Galveston, TX, USA.
³ Center for Biodefense and Emerging Infectious Diseases and Department of Pathology, University of Texas Medical Branch, Galveston, TX, USA.
⁴ US Naval Medical Research Unit no. 6, Lima, Peru.
⁵ Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, TX, USA.
⁶ Center for Vaccine Research, Department of Computational and Systems Biology, University of Pittsburgh, Pittsburgh, PA, USA.
⁷ Naval Medical Research Center, Silver Spring, MD, USA.
⁸ Marie Bashir Institute for Infectious Diseases and Biosecurity, School of Biological Sciences and Sydney Medical School, University of Sydney, Sydney, NSW 2006, Australia.
⁹ CSIRO Animal, Food and Health Sciences, Australian Animal Health Laboratory, Geelong, VIC 3220, Australia.

Abstract

Arboretum virus (ABTV) and Puerto Almendras virus (PTAMV) are two mosquito-associated rhabdoviruses isolated from pools of Psorophora albigenu and Ochlerotattus fulvus mosquitoes, respectively, collected in the Department of Loreto, Peru, in 2009. Initial tests suggested that both viruses were novel rhabdoviruses and this was confirmed by complete genome sequencing. Analysis of their 11 482 nt (ABTV) and 11 876 (PTAMV) genomes indicates that they encode the five canonical rhabdovirus structural proteins (N, P, M, G and L) with an additional gene (U1) encoding a small hydrophobic protein. Evolutionary analysis of the L protein indicates that ABTV and PTAMV are novel and phylogenetically distinct rhabdoviruses that cannot be classified as members of any of the eight currently recognized genera within the family Rhabdoviridae, highlighting the vast diversity of this virus family.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Animals
Cluster Analysis
Culicidae / virology*
Female
Genome, Viral*
Microscopy, Electron, Transmission
Molecular Sequence Data
Open Reading Frames
Peru
Phylogeny
RNA, Viral / genetics*
Rhabdoviridae / classification*
Rhabdoviridae / genetics
Rhabdoviridae / isolation & purification*
Sequence Analysis, DNA*
Sequence Homology
Viral Proteins / genetics
Virion / ultrastructure

Arboretum and Puerto Almendras viruses: two novel rhabdoviruses isolated from mosquitoes in Peru

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