Candida nivariensis isolated from a renal transplant patient with persistent candiduria-Molecular identification using ITS PCR and MALDI-TOF

Rebecca L Gorton; Gareth L Jones; Christopher C Kibbler; Sophie Collier

doi:10.1016/j.mmcr.2013.10.001

Candida nivariensis isolated from a renal transplant patient with persistent candiduria-Molecular identification using ITS PCR and MALDI-TOF

Med Mycol Case Rep. 2013 Oct 25:2:156-8. doi: 10.1016/j.mmcr.2013.10.001.

Authors

Rebecca L Gorton¹, Gareth L Jones², Christopher C Kibbler¹, Sophie Collier¹

Affiliations

¹ Department of Microbiology, Royal Free Hospital, Pond Street, London NW3 2QG, United Kingdom.
² UCL Centre for Nephrology, Royal Free Hospital, Pond Street, London NW3 2QG, United Kingdom.

Abstract

We report on the isolation of Candida nivariensis from a renal transplant patient with persistent candiduria. Biochemical profiling misidentified isolates as Candida glabrata (3/5) and Candida inconspicua (2/5). All isolates produced white colonies on CHROMagar(™) Candida medium. Internal transcribed spacer (ITS) ribosomal gene sequence analysis and MALDI-TOF-MS analysis (Bruker Biotyper(™) 2.0) identified all isolates as C. nivariensis, demonstrating the utility of MALDI-TOF as a rapid, accurate approach for the identification of cryptic Candida species.

Keywords: C. nivariensis; Candiduria; ITS; MALDI-TOF.