Detection of chromosomal bla(CTX-M-2) in diverse Escherichia coli isolates from healthy broiler chickens

Clin Microbiol Infect. 2014 Oct;20(10):O623-6. doi: 10.1111/1469-0691.12531. Epub 2014 Feb 20.

Abstract

The rise of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli in food-producing animals is a growing concern for public health. We investigated ESBL producers isolated from broiler chickens in Brazil and characterized 19 CTX-M-2-producing E. coli. The ISCR1 was detected upstream of the chromosome-located gene bla(CTX-M-2), associated with sul-1 type integron structure. CTX-M-2-producing E. coli exhibited different PFGE-types and phylogenetic groups, showing a non-clonal dissemination. The sequence types found (ST93, ST155 and ST2309) have been associated with humans and animals worldwide. Herein, we report the chromosomal location of bla(CTX-M-2) on E. coli, highlighting the risks of multidrug-resistant bacteria in food-producing animals.

Keywords: Brazil; extended-spectrum beta-lactamase; food- producing animals; poultry; qnr.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brazil
  • Chickens*
  • Chromosomes, Bacterial
  • Drug Resistance, Multiple, Bacterial
  • Escherichia coli / enzymology
  • Escherichia coli / genetics
  • Escherichia coli / isolation & purification*
  • Escherichia coli Infections / microbiology
  • Escherichia coli Infections / veterinary*
  • Evolution, Molecular
  • Humans
  • Phylogeny
  • Poultry Diseases / microbiology*
  • beta-Lactamases / genetics*
  • beta-Lactamases / metabolism

Substances

  • beta-lactamase CTX-2
  • beta-Lactamases