Knowledge of animal mitochondrial genomes is very important to understand their molecular evolution and phylogenetic studies. Cobitis granoei (Rendahl, 1935) only had been sequenced D-loop gene and Cytb gene in past research. Here we determined the complete mitochondrial genome of C. granoei. The entire sequence was 16,636 bp in length, including 13 protein-coding genes (PCGs), 2 rRNA genes, 22 tRNA genes and one control region (D-loop). All the PCGs used ATG as a start codon, except for COI gene, which with GTG as the start codon. Most of the PCGs, including ND1, COI, ATP8, ATP6, ND4L, ND5 and ND6 had TAA as termination codon. While the others were with the incomplete termination codons TA or T. The gene arrangement was in accordance with all known Cyprinformes mitochondrial genomes.
Keywords: Cobitidae; Cobitis granoei; Cyprinformes; mitochondrial genome.