Discovery of C-(1-aryl-cyclohexyl)-methylamines as selective, orally available inhibitors of dipeptidyl peptidase IV

Bioorg Med Chem Lett. 2014 Feb 1;24(3):731-6. doi: 10.1016/j.bmcl.2013.12.118. Epub 2014 Jan 6.

Abstract

The successful launches of dipeptidyl peptidase IV (DPP IV) inhibitors as oral anti-diabetics warrant and spur the further quest for additional chemical entities in this promising class of therapeutics. Numerous pharmaceutical companies have pursued their proprietary candidates towards the clinic, resulting in a large body of published chemical structures associated with DPP IV. Herein, we report the discovery of a novel chemotype for DPP IV inhibition based on the C-(1-aryl-cyclohexyl)-methylamine scaffold and its optimization to compounds which selectively inhibit DPP IV at low-nM potency and exhibit an excellent oral pharmacokinetic profile in the rat.

Keywords: Dipeptidyl peptidase IV; Enzyme selectivity; Ex vivo plasma DPP IV inhibition; Pharmacokinetics; Protease inhibition; Structure-based drug design.

MeSH terms

  • Adamantane / analogs & derivatives
  • Adamantane / chemistry
  • Adamantane / pharmacology
  • Administration, Oral
  • Animals
  • Caco-2 Cells
  • Crystallography, X-Ray
  • Cyclization
  • Dipeptidyl Peptidase 4 / metabolism*
  • Dipeptidyl-Peptidase IV Inhibitors / chemical synthesis*
  • Dipeptidyl-Peptidase IV Inhibitors / chemistry
  • Dipeptidyl-Peptidase IV Inhibitors / pharmacokinetics*
  • Dipeptidyl-Peptidase IV Inhibitors / pharmacology
  • Drug Discovery*
  • Enzyme Activation / drug effects
  • Humans
  • Inhibitory Concentration 50
  • Methylamines / chemical synthesis*
  • Methylamines / chemistry
  • Methylamines / pharmacokinetics*
  • Methylamines / pharmacology
  • Molecular Structure
  • Nitriles / chemistry
  • Nitriles / pharmacology
  • Pyrazines / chemistry
  • Pyrazines / pharmacology
  • Pyrrolidines / chemistry
  • Pyrrolidines / pharmacology
  • Rats
  • Sitagliptin Phosphate
  • Triazoles / chemistry
  • Triazoles / pharmacology
  • Vildagliptin

Substances

  • Dipeptidyl-Peptidase IV Inhibitors
  • Methylamines
  • Nitriles
  • Pyrazines
  • Pyrrolidines
  • Triazoles
  • DPP4 protein, human
  • Dipeptidyl Peptidase 4
  • Vildagliptin
  • Adamantane
  • Sitagliptin Phosphate