Augmentation of the antibody response in aged mice with an 8-derivatized guanosine nucleoside and its effect on immunological memory

Cell Immunol. 1987 Oct 15;109(2):332-7. doi: 10.1016/0008-8749(87)90316-9.

Abstract

The injection of aged mice with 7-methyl-8-oxoguanosine (7m8oGuo) along with aggregated human gamma-globulin (AHGG) results in an enhanced anti-HGG antibody response. Aged mice injected with AHGG alone make only a feeble anti-HGG response. The enhanced response in aged mice was not significantly different from the response obtained in young-adult mice injected only with AHGG. However, the enhanced response obtained by injection of 7m8oGuo in young-adult mice was several orders of magnitude greater than that observed in the aged mice. Aged mice given a primary injection of AHGG alone failed to make a secondary response to AHGG given 90 days later, whereas aged mice injected with 7m8oGuo along with a primary injection of AHGG showed variable secondary antibody responses to AHGG. Two of seven mice showed high degrees of immunological memory equivalent to that seen in young-adult mice while others showed either meagre or no responses. The effect of 7m8oGuo appeared to be primarily on B cells, albeit the role of T cells cannot be ruled out.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Aging
  • Animals
  • Antibody Formation / drug effects*
  • Female
  • Guanosine / analogs & derivatives*
  • Guanosine / pharmacology
  • Immunologic Memory / drug effects*
  • Mice
  • Mice, Inbred CBA
  • gamma-Globulins / immunology

Substances

  • gamma-Globulins
  • Guanosine
  • 7-methyl-8-oxoguanosine