Evaluation of BMPA, MWY, GVPC and BCYE media for the isolation of Legionella species from respiratory samples

G Descours; P Cassier; F Forey; C Ginevra; J Etienne; G Lina; S Jarraud

doi:10.1016/j.mimet.2014.01.001

Evaluation of BMPA, MWY, GVPC and BCYE media for the isolation of Legionella species from respiratory samples

J Microbiol Methods. 2014 Mar:98:119-21. doi: 10.1016/j.mimet.2014.01.001. Epub 2014 Jan 23.

Authors

G Descours¹, P Cassier², F Forey³, C Ginevra⁴, J Etienne⁴, G Lina⁴, S Jarraud⁴

Affiliations

¹ CIRI, International Center for Infectiology Research, Université de Lyon, Lyon, France; INSERM U1111, Lyon, France; Ecole Normale Supérieure de Lyon, Lyon, France; Université Lyon 1, Centre International de Recherche en Infectiologie, Lyon, France; CNRS, UMR5308, Lyon, France; Hospices Civils de Lyon, Groupement Hospitalier Est, Legionella National Reference Center, East Biology & Pathology Center, 59 Boulevard Pinel, 69677 Bron Cedex, France. Electronic address: [email protected].
² CIRI, International Center for Infectiology Research, Université de Lyon, Lyon, France; INSERM U1111, Lyon, France; Ecole Normale Supérieure de Lyon, Lyon, France; Université Lyon 1, Centre International de Recherche en Infectiologie, Lyon, France; CNRS, UMR5308, Lyon, France; Hospices Civils de Lyon, Groupement Hospitalier Est, Legionella National Reference Center, East Biology & Pathology Center, 59 Boulevard Pinel, 69677 Bron Cedex, France; Hospices Civils de Lyon, Edouard Herriot Hospital, Infection Control Unit, 5 Place d'Arsonval, 69437 Lyon Cedex 03, France.
³ Hospices Civils de Lyon, Groupement Hospitalier Est, Legionella National Reference Center, East Biology & Pathology Center, 59 Boulevard Pinel, 69677 Bron Cedex, France.
⁴ CIRI, International Center for Infectiology Research, Université de Lyon, Lyon, France; INSERM U1111, Lyon, France; Ecole Normale Supérieure de Lyon, Lyon, France; Université Lyon 1, Centre International de Recherche en Infectiologie, Lyon, France; CNRS, UMR5308, Lyon, France; Hospices Civils de Lyon, Groupement Hospitalier Est, Legionella National Reference Center, East Biology & Pathology Center, 59 Boulevard Pinel, 69677 Bron Cedex, France.

PMID: 24462808
DOI: 10.1016/j.mimet.2014.01.001

Abstract

Culture media performance is a critical factor in the isolation of Legionellae from respiratory samples. We showed that BMPA and MWY media yielded significantly higher isolation rates than GVPC and BCYE media in regard to performance with samples that harbored low Legionella inocula and high contamination levels.

Keywords: BMPA; GVPC; Isolation; Legionella; MWY; Respiratory samples.

MeSH terms

Culture Media / metabolism*
Humans
Legionella / isolation & purification*
Respiratory System / microbiology*

Substances

Culture Media