Abstract
At the onset of mitosis, the centrosome undergoes maturation, which is characterized by a drastic expansion of the pericentriolar material (PCM) and a robust increase in microtubule-organizing activity. CEP215 is one of the major PCM components which accumulates at the centrosome during mitosis. The depletion phenotypes indicate that CEP215 is essential for centrosome maturation and bipolar spindle formation. Here, we performed a series of knockdown-rescue experiments to link the protein-protein interaction properties of CEP215 to its biological functions. The results showed that CEP215 and pericentrin, another major PCM component, is interdependent for their accumulation at the spindle poles during mitosis. As a result, The CEP215-pericentrin interaction is required for centrosome maturation and subsequent bipolar spindle formation during mitosis. On the other hand, CEP215 interaction with γ-tubulin is dispensable for centrosome maturation. Our results provide an insight how PCM components are assembled to form a spindle pole during mitosis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antigens / chemistry
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Antigens / metabolism*
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Cell Cycle Proteins
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Centrosome / metabolism
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Centrosome / physiology*
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HEK293 Cells
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HeLa Cells
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Humans
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Immunoblotting
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Immunohistochemistry
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Immunoprecipitation
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Intracellular Signaling Peptides and Proteins / metabolism*
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Microscopy, Fluorescence
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Mitosis / physiology*
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Models, Molecular*
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Nerve Tissue Proteins / metabolism*
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RNA, Small Interfering / genetics
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Spindle Apparatus / genetics
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Spindle Apparatus / physiology
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Tubulin
Substances
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Antigens
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CDK5RAP2 protein, human
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Cell Cycle Proteins
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Intracellular Signaling Peptides and Proteins
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Nerve Tissue Proteins
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RNA, Small Interfering
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Tubulin
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pericentrin
Grants and funding
This study was supported by grants from the BioImaging Research Center at GIST; the Basic Research Program (Grant Number 2012R1A2A201003512) and the Science Research Center Program (Grant Number 2011-0006425). Seongjae Kim was supported by the Brain Korea 21 Project. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.