[Gene detection of GPD1-L and the association with sudden unexplained death syndrome in young adults]

Fa Yi Xue Za Zhi. 2013 Oct;29(5):348-52.
[Article in Chinese]

Abstract

Objective: To analyze the variations of glycerol-3-phosphate dehydrogenase 1 like gene (GPD1-L) and address the association with sudden manhood death syndrome (SMDS).

Methods: The genomic DNA was extracted from blood samples of the SMDS group and the normal control group. The exons, exon-intron boundaries and 3'-UTRs of coding region of GPD1-L were PCR amplified and DNA sequenced directly to confirm the types of variations. The genotype frequency and allele frequency were analyzed statistically.

Results: There were two variants in the SMDS group, c.465C>T and c.*18G>T, the latter existed certain degree difference of genotype distribution and allele frequency between the SMDS group and the control group, but there was no statistically significant (P > 0.05).

Conclusion: The relation between gene mutation of GPD1-L and the occurrence of Chinese SMDS deserves a further research.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Asian People / genetics
  • Base Sequence
  • Case-Control Studies
  • DNA Mutational Analysis
  • DNA Primers / genetics
  • Death, Sudden / etiology*
  • Exons
  • Gene Frequency
  • Genotype
  • Glycerolphosphate Dehydrogenase / blood
  • Glycerolphosphate Dehydrogenase / genetics*
  • Humans
  • Male
  • Middle Aged
  • Mutation*
  • Polymerase Chain Reaction
  • Polymorphism, Single Nucleotide
  • Young Adult

Substances

  • DNA Primers
  • GPD1L protein, human
  • Glycerolphosphate Dehydrogenase