Isolation of cDNAs coding for epitopes shared by microtubule-associated proteins and neurofibrillary tangle in Alzheimer's disease

FEBS Lett. 1987 Dec 21;226(1):28-32. doi: 10.1016/0014-5793(87)80544-6.

Abstract

5 cross-hybridizing cDNA clones of sizes 2.2 (3 cDNAs), 1.3 and 0.8 kb corresponding to tau microtubule-associated protein have been isolated from a rat brain lambda gt11 expression library. Antibodies affinity-purified using the fusion protein encoded by the cDNAs were observed to label tau polypeptides on Western blots and Alzheimer's neurofibrillary tangles.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alzheimer Disease / metabolism*
  • Alzheimer Disease / pathology
  • Animals
  • Brain / pathology*
  • Cloning, Molecular
  • DNA / isolation & purification*
  • Epitopes / analysis
  • Epitopes / genetics*
  • Humans
  • Microtubule-Associated Proteins / genetics*
  • Microtubule-Associated Proteins / immunology
  • Nerve Tissue Proteins / genetics*
  • Nerve Tissue Proteins / immunology
  • Rats
  • tau Proteins

Substances

  • Epitopes
  • Microtubule-Associated Proteins
  • Nerve Tissue Proteins
  • tau Proteins
  • DNA