Evaluation of a short, on-plate formic acid extraction method for matrix-assisted laser desorption ionization-time of flight mass spectrometry-based identification of clinically relevant yeast isolates

Rebecca L Gorton; Shila Seaton; Purnima Ramnarain; Timothy D McHugh; Christopher C Kibbler

doi:10.1128/JCM.03489-13

Evaluation of a short, on-plate formic acid extraction method for matrix-assisted laser desorption ionization-time of flight mass spectrometry-based identification of clinically relevant yeast isolates

J Clin Microbiol. 2014 Apr;52(4):1253-5. doi: 10.1128/JCM.03489-13. Epub 2014 Jan 29.

Authors

Rebecca L Gorton¹, Shila Seaton, Purnima Ramnarain, Timothy D McHugh, Christopher C Kibbler

Affiliation

¹ UK Clinical Mycology Network (UKCMN) Regional Laboratory, Department of Medical Microbiology, Royal Free Hospital, Pond Street, Hampstead, London, United Kingdom.

Abstract

This report describes a short, on-plate formic acid (FA) extraction method for the identification of clinical yeast isolates using matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS). A total of 41.1% (78/190) and 63.7% (121/190) of yeasts were identified using species log score thresholds of >2.0 and >1.9, respectively. Overall, 97.4% (185/190) of yeasts were identified in combination with conventional FA extraction.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Formates
Humans
Mycoses / diagnosis*
Sensitivity and Specificity
Specimen Handling / methods*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
Yeasts / chemistry*
Yeasts / classification*
Yeasts / isolation & purification

Substances

Formates
formic acid