MiR-361-5p acts as a tumor suppressor in prostate cancer by targeting signal transducer and activator of transcription-6(STAT6)

Dachuang Liu; Tao Tao; Bin Xu; Shuqiu Chen; Chunhui Liu; Lei Zhang; Kai Lu; Yeqing Huang; Liang Jiang; Xiaowen Zhang; Xiaoming Huang; Lihua Zhang; Conghui Han; Ming Chen

doi:10.1016/j.bbrc.2014.01.140

MiR-361-5p acts as a tumor suppressor in prostate cancer by targeting signal transducer and activator of transcription-6(STAT6)

Biochem Biophys Res Commun. 2014 Feb 28;445(1):151-6. doi: 10.1016/j.bbrc.2014.01.140. Epub 2014 Jan 31.

Authors

Dachuang Liu¹, Tao Tao², Bin Xu², Shuqiu Chen², Chunhui Liu², Lei Zhang³, Kai Lu³, Yeqing Huang², Liang Jiang², Xiaowen Zhang³, Xiaoming Huang⁴, Lihua Zhang⁵, Conghui Han⁶, Ming Chen⁷

Affiliations

¹ Department of Urology, Zhongda Hospital, Medical School, Southeast University, Nanjing, Jiangsu Province 210009, China; Department of Urology, Xuzhou Central Hospital Affiliated with Southeast University, Xuzhou, Jiangsu Province 221009, China; Surgery Central Laboratory of Southeast University, Nanjing, Jiangsu Province 210009, China.
² Department of Urology, Zhongda Hospital, Medical School, Southeast University, Nanjing, Jiangsu Province 210009, China; Surgery Central Laboratory of Southeast University, Nanjing, Jiangsu Province 210009, China.
³ Department of Urology, Zhongda Hospital, Medical School, Southeast University, Nanjing, Jiangsu Province 210009, China.
⁴ Surgery Central Laboratory of Southeast University, Nanjing, Jiangsu Province 210009, China.
⁵ Department of Pathology, Zhongda Hospital Affiliated with Southeast University, Nanjing, Jiangsu Province 210009, China.
⁶ Department of Urology, Xuzhou Central Hospital Affiliated with Southeast University, Xuzhou, Jiangsu Province 221009, China.
⁷ Department of Urology, Zhongda Hospital, Medical School, Southeast University, Nanjing, Jiangsu Province 210009, China; Surgery Central Laboratory of Southeast University, Nanjing, Jiangsu Province 210009, China. Electronic address: [email protected].

PMID: 24491557
DOI: 10.1016/j.bbrc.2014.01.140

Abstract

Castration-resistant prostate cancer (CRPC), whose pathogenesis is known to be regulated by microRNAs (miRNAs), has a poor prognosis. In our present study, we found that the expression of miR-361-5p in CRPC was lower than in androgen-dependent prostate cancer (ADPC), indicating that miR-361-5p may play an important role in the progression of ADPC to CRPC. The role of miR-361-5p in prostate cancer (PCa) has not been evaluated until date. Our findings suggest that miR-361-5p is a suppressor in CRPC. Signal transducer and activator of transcription-6 (STAT6), a direct target of miR-361-5p, enhances the expression of B-cell lymphoma-extra large (Bcl-xL), while miR-361-5p inhibits its expression through STAT6. Therefore, miR-361-5p has great clinical significance in preventing the malignant progression of PCa.

Keywords: Bcl-xL; Castration-resistant prostate cancer (CRPC); STAT6; miR-361-5p.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

3' Untranslated Regions / genetics
Animals
Blotting, Western
Cell Line, Tumor
Cell Proliferation
Gene Expression Profiling
Gene Expression Regulation, Neoplastic
Humans
Male
Mice
Mice, Inbred BALB C
Mice, Nude
MicroRNAs / genetics*
Mutation
Oligonucleotide Array Sequence Analysis
Prostatic Neoplasms / genetics*
Prostatic Neoplasms / metabolism
Prostatic Neoplasms / pathology
Prostatic Neoplasms, Castration-Resistant / genetics
Prostatic Neoplasms, Castration-Resistant / metabolism
Prostatic Neoplasms, Castration-Resistant / pathology
RNA Interference
Reverse Transcriptase Polymerase Chain Reaction
STAT6 Transcription Factor / genetics*
STAT6 Transcription Factor / metabolism
Tumor Suppressor Proteins / genetics*
Xenograft Model Antitumor Assays
bcl-X Protein / genetics
bcl-X Protein / metabolism

Substances

3' Untranslated Regions
MIRN361 microRNA, human
MicroRNAs
STAT6 Transcription Factor
Tumor Suppressor Proteins
bcl-X Protein