Comparison of the effects of Mg-6Zn and Ti-3Al-2.5V alloys on TGF-β/TNF-α/VEGF/b-FGF in the healing of the intestinal tract in vivo

Biomed Mater. 2014 Apr;9(2):025011. doi: 10.1088/1748-6041/9/2/025011. Epub 2014 Feb 11.

Abstract

To evaluate the different effects of Mg-6Zn alloy and Ti-3Al-2.5V alloy implants in intestinal tract healing, we compared these two different alloys with respect to their effect on a rat's intestinal tract, using serum magnesium, radiology, pathology and immunohistochemistry in vivo. It was found using the scanning electron microscope that the Mg-6Zn alloy began to degrade during the first week and that the Ti-3Al-2.5V alloy was non-degradable throughout the process. The Mg-6Zn alloy did not have an impact on serum magnesium. Superior to the Ti-3Al-2.5V alloy, the Mg-6Zn alloy enhanced the expression of transforming growth factor-β1 in healing tissue, and promoted the expression of both the vascular endothelial growth factor and the basic fibroblast growth factor, which helped angiogenesis and healing. The Mg-6Zn alloy reduced the expression of the tumor necrosis factor (TNF-α) at different stages and decreased inflammatory response, which may have been related to the zinc inhibiting TNF-α. In general, the Mg-6Zn alloy performed better than Ti-3Al-2.5V at promoting healing and reducing inflammation. The Mg-6Zn alloy may be a promising candidate for use in the pins of circular staplers for gastrointestinal reconstruction in medicine.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alloys / chemistry*
  • Aluminum / chemistry
  • Animals
  • Biocompatible Materials / chemistry
  • Fibroblast Growth Factor 2 / metabolism*
  • Inflammation
  • Intestinal Mucosa / metabolism*
  • Magnesium / chemistry
  • Male
  • Materials Testing
  • Microscopy, Electron, Scanning
  • Rats
  • Rats, Sprague-Dawley
  • Titanium / chemistry
  • Transforming Growth Factor beta1 / metabolism*
  • Tumor Necrosis Factor-alpha / metabolism*
  • Vanadium / chemistry
  • Vascular Endothelial Growth Factor A / metabolism*
  • Wound Healing
  • Zinc / chemistry

Substances

  • Alloys
  • Biocompatible Materials
  • Mg-6Zn alloy
  • Tgfb1 protein, rat
  • Ti-3Al-2.5V alloy
  • Transforming Growth Factor beta1
  • Tumor Necrosis Factor-alpha
  • Vascular Endothelial Growth Factor A
  • vascular endothelial growth factor A, rat
  • Vanadium
  • Fibroblast Growth Factor 2
  • Aluminum
  • Titanium
  • Magnesium
  • Zinc