Functional rectification of the newly described African henipavirus fusion glycoprotein (Gh-M74a)

J Virol. 2014 May;88(9):5171-6. doi: 10.1128/JVI.03655-13. Epub 2014 Feb 12.

Abstract

Recent evidence identified multiple Henipavirus species in Africa distinct from those in Southeast Asia and Australia. The reported fusion glycoprotein (F) sequence of the African Gh-M74a strain (GhV-F) is likely incorrect: a single base pair deletion near the N terminus results in multiple aberrancies. Rectifying this by adding single nucleotide insertions results in a GhV-F that now possesses a signal peptide, is efficiently cell surface expressed, exhibits syncytium formation when coexpressed with GhV-G protein, and mediates pseudotyped viral particle entry.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Africa
  • Amino Acid Sequence
  • Base Sequence
  • Henipavirus / genetics*
  • Henipavirus / physiology*
  • Humans
  • Molecular Sequence Data
  • Sequence Alignment
  • Viral Fusion Proteins / genetics*
  • Viral Fusion Proteins / metabolism*
  • Virus Internalization*

Substances

  • Viral Fusion Proteins