Structural and genetic studies of the O-antigen of Enterobacter cloacae G2277

Carbohydr Res. 2014 Mar 31:387:10-3. doi: 10.1016/j.carres.2014.01.001. Epub 2014 Jan 14.

Abstract

The O-polysaccharide was isolated by mild acid degradation of the lipopolysaccharide of Enterobacter cloacae G2277 and studied by sugar analysis along with 1D and 2D (1)H and (13)C NMR spectroscopy. The following structure of the linear pentasaccharide repeating unit was established, where a galacturonic acid (GalA) residue is mono-O-acetylated at position either 2 or 3: The O-antigen gene cluster of E. cloacae G2277 was sequenced. The gene functions were tentatively assigned by comparison with sequences in the available databases and found to be in agreement with the O-polysaccharide structure.

Keywords: Bacterial polysaccharide structure; Enterobacter cloacae; Lipopolysaccharide; O-Antigen gene cluster; O-Polysaccharide.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylation
  • Carbohydrate Sequence
  • Enterobacter cloacae / chemistry*
  • Lipopolysaccharides / chemistry
  • Magnetic Resonance Spectroscopy
  • Molecular Structure
  • Multigene Family
  • O Antigens / chemistry*
  • O Antigens / genetics*

Substances

  • Lipopolysaccharides
  • O Antigens