Some properties of inhibitory action of lidocaine on the Ca2+ current of single isolated frog sensory neurons

Brain Res. 1988 Mar 1;442(2):223-8. doi: 10.1016/0006-8993(88)91507-7.

Abstract

The action of lidocaine on the Ca2+ current (ICa) was studied on single isolated neurons of frog dorsal root ganglia using a 'concentration-clamp' technique which combines intracellular perfusion with a rapid external solution change. Lidocaine decreased the peak amplitude of ICa at a threshold concentration of 10 microM. Higher concentrations gave a dose-dependent increase in inhibition of ICa. Lidocaine also depressed the Na+ current (INa) at a threshold concentration 10 times lower than that for decreasing the amplitude of ICa of neurons isolated from same ganglia. The rate of inhibitory action on ICa was slowed at more negative holding potentials (VH). Lidocaine appears to block both closed and open Ca2+ channels, but acts more profoundly on open channels.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Calcium / metabolism*
  • Calcium / pharmacology
  • Ganglia, Spinal / physiology
  • In Vitro Techniques
  • Ion Channels / drug effects
  • Ion Channels / physiology*
  • Kinetics
  • Lidocaine / pharmacology*
  • Membrane Potentials / drug effects
  • Neurons, Afferent / drug effects
  • Neurons, Afferent / physiology*
  • Rana catesbeiana

Substances

  • Ion Channels
  • Lidocaine
  • Calcium