Abstract
A simple, efficient, and scalable manufacturing technique is required for developing siRNA-lipid nanoparticles (siRNA-LNP) for therapeutic applications. In this chapter we describe a novel microfluidic-based manufacturing process for the rapid manufacture of siRNA-LNP, together with protocols for characterizing the size, polydispersity, RNA encapsulation efficiency, RNA concentration, and total lipid concentration of the resultant nanoparticles.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cholesterol / chemistry*
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Drug Compounding / instrumentation
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Drug Compounding / methods
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Drug Delivery Systems / instrumentation
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Drug Delivery Systems / methods*
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Humans
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Microfluidics / instrumentation*
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Nanoparticles / chemistry*
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Particle Size
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Phosphatidylcholines / chemistry*
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RNA, Small Interfering / chemistry*
Substances
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Phosphatidylcholines
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RNA, Small Interfering
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Cholesterol
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1,2-distearoyllecithin