A new era of semiconductor genetics using ion-sensitive field-effect transistors: the gene-sensitive integrated cell

Philos Trans A Math Phys Eng Sci. 2014 Feb 24;372(2012):20130112. doi: 10.1098/rsta.2013.0112. Print 2014 Mar 28.

Abstract

Semiconductor genetics is now disrupting the field of healthcare owing to the rapid parallelization and scaling of DNA sensing using ion-sensitive field-effect transistors (ISFETs) fabricated using commercial complementary metal -oxide semiconductor technology. The enabling concept of DNA reaction monitoring introduced by Toumazou has made this a reality and we are now seeing relentless scaling with Moore's law ultimately achieving the $100 genome. In this paper, we present the next evolution of this technology through the creation of the gene-sensitive integrated cell (GSIC) for label-free real-time analysis based on ISFETs. This device is derived from the traditional metal-oxide semiconductor field-effect transistor (MOSFET) and has electrical performance identical to that of a MOSFET in a standard semiconductor process, yet is capable of incorporating DNA reaction chemistries for applications in single nucleotide polymorphism microarrays and DNA sequencing. Just as application-specific integrated circuits, which are developed in much the same way, have shaped our consumer electronics industry and modern communications and memory technology, so, too, do GSICs based on a single underlying technology principle have the capacity to transform the life science and healthcare industries.

Keywords: DNA; ISFET; complementary metal-oxide semiconductor; genetics; ion-sensitive field-effect transistor.

MeSH terms

  • Biosensing Techniques / instrumentation
  • Computational Biology / methods
  • DNA / analysis
  • Electronics
  • Equipment Design
  • Humans
  • Ions*
  • Metals / chemistry
  • Oligonucleotide Array Sequence Analysis
  • Oxides / chemistry
  • Polymorphism, Single Nucleotide
  • Semiconductors*
  • Sequence Analysis, DNA / economics
  • Sequence Analysis, DNA / instrumentation*

Substances

  • Ions
  • Metals
  • Oxides
  • DNA