Enhancement of frameshift mutagenesis in Salmonella typhimurium derivatives of hisC3076 by 5-azacytidine and other agents

Mutagenesis. 1986 Jul;1(4):283-6. doi: 10.1093/mutage/1.4.283.

Abstract

The yield of frameshift revertants of Salmonella typhimurium strain hisC3076 on plates containing the mutagen 9-aminoacridine (9AA) is enhanced by the addition to the selection medium of a number of chemical agents. These include 5-azacytidine (5-azaC), naladixic acid, ethyl methanesulphonate; pre-treatment of cells with u.v. light or gamma-radiation is also effective. With the exception of u.v. which is detected as a poor mutagen in strain hisC3076, all other enhancing agents are not usually detected as mutagens in this strain. The observed synergism between 9AA and 5-azaC in recA and uvrB derivatives of hisC3076 eliminates the possibility that recA-dependent repair or excision repair is necessary. However a lack of synergism in a uvrD derivative suggests that helicase II is involved. It is suggested that the presence of 9AA in the plating medium enables the synergistic agents to be detected as mutagens.

MeSH terms

  • Aminacrine / adverse effects
  • Azacitidine / adverse effects*
  • DNA, Bacterial / drug effects
  • Drug Synergism
  • Ethyl Methanesulfonate / adverse effects
  • Mutagenicity Tests*
  • Mutation
  • Salmonella typhimurium

Substances

  • DNA, Bacterial
  • Aminacrine
  • Ethyl Methanesulfonate
  • Azacitidine