Whole genome analysis of a community-associated methicillin-resistant Staphylococcus aureus ST59 isolate from a case of human sepsis and severe pneumonia in China

Tingting Qu; Ye Feng; Yan Jiang; Peiqiong Zhu; Zeqing Wei; Yan Chen; Michael Otto; Yunsong Yu

doi:10.1371/journal.pone.0089235

Whole genome analysis of a community-associated methicillin-resistant Staphylococcus aureus ST59 isolate from a case of human sepsis and severe pneumonia in China

PLoS One. 2014 Feb 20;9(2):e89235. doi: 10.1371/journal.pone.0089235. eCollection 2014.

Authors

Tingting Qu¹, Ye Feng², Yan Jiang², Peiqiong Zhu³, Zeqing Wei¹, Yan Chen², Michael Otto⁴, Yunsong Yu²

Affiliations

¹ State Key Laboratory for Diagnosis and Treatment of Infectious Disease, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, China.
² Department of Infectious Diseases, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, China.
³ Department of Clinical Medicine, Zhejiang Medical College, Hangzhou, Zhejiang, China.
⁴ Pathogen Molecular Genetics Section, Laboratory of Human Bacterial Pathogenesis, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America.

Abstract

We report a case of necrotizing pneumonia in a young patient caused by community acquired-methicillin resistant Staphylococcus aureus (CA-MRSA) in a teaching hospital in the People's Republic of China. The patient had a typical clinical presentation and was successfully treated with antibiotics and intravenous immunoglobulin. A CA-MRSA strain, named SA268, was isolated from the blood of the patient. The isolate was susceptible to most antimicrobial agents, except cephalosporins, penicillins, and β-lactamase inhibitor combinations. Multi-locus sequence typing (MLST) assigned SA268 to ST59, a clone widely spread in eastern Asia. The strain was positive for Panton Valentine Leukocidin (PVL)-encoding genes and SCCmec type V. We sequenced the complete genome of the SA268 isolate. The genome of SA268 was almost identical to that of the Taiwanese ST59 CA-MRSA strains M013 and SA957. However, we observed several differences in gene composition, which included differences in the SCCmec element and several lipoprotein genes that were present in the Taiwanese strains but absent from SA268.

Publication types

Case Reports
Comparative Study
Research Support, N.I.H., Intramural
Research Support, Non-U.S. Gov't

MeSH terms

Adolescent
Anti-Bacterial Agents / administration & dosage
China
Community-Acquired Infections / drug therapy
Community-Acquired Infections / genetics*
Community-Acquired Infections / microbiology
DNA, Bacterial / genetics
Genome, Bacterial / genetics*
Humans
Immunoglobulins, Intravenous / administration & dosage
Male
Methicillin-Resistant Staphylococcus aureus / drug effects
Methicillin-Resistant Staphylococcus aureus / genetics*
Methicillin-Resistant Staphylococcus aureus / isolation & purification
Microbial Sensitivity Tests
Multilocus Sequence Typing
Phylogeny
Pneumonia, Staphylococcal / drug therapy
Pneumonia, Staphylococcal / genetics*
Pneumonia, Staphylococcal / microbiology
Sepsis / drug therapy
Sepsis / genetics*
Sepsis / microbiology
Sequence Analysis, DNA
Staphylococcal Infections / drug therapy
Staphylococcal Infections / genetics*
Staphylococcal Infections / microbiology

Substances

Anti-Bacterial Agents
DNA, Bacterial
Immunoglobulins, Intravenous

Grants and funding

Intramural NIH HHS/United States