Expression and solubility testing in a high-throughput environment

Keehwan Kwon; Scott N Peterson

doi:10.1007/978-1-4939-0354-2_6

Expression and solubility testing in a high-throughput environment

Methods Mol Biol. 2014:1140:75-88. doi: 10.1007/978-1-4939-0354-2_6.

Authors

Keehwan Kwon¹, Scott N Peterson

Affiliation

¹ J. Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD, 20850, USA, [email protected].

PMID: 24590710
DOI: 10.1007/978-1-4939-0354-2_6

Abstract

The expression and screening of the solubility of recombinant proteins is an important step in the high-throughput (HT) production of target proteins. For many applications, E. coli remains the most widely used expression system due to the relative ease of adapting it to HT pipelines. Herein is described a platform using a 96-well format for efficient expression and solubility screening of target proteins.

Publication types

Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Gene Expression Regulation
High-Throughput Screening Assays / methods*
Molecular Biology / methods*
Recombinant Proteins / biosynthesis*
Recombinant Proteins / genetics
Solubility
Structural Homology, Protein

Substances

Recombinant Proteins

Abstract

Publication types

MeSH terms

Substances

Grants and funding