Longitudinal evaluation of enteric protozoa in Haitian children by stool exam and multiplex serologic assay

Am J Trop Med Hyg. 2014 Apr;90(4):653-60. doi: 10.4269/ajtmh.13-0545. Epub 2014 Mar 3.

Abstract

Haitian children were monitored longitudinally in a filariasis study. Included were stool samples examined for Giardia intestinalis and Entamoeba histolytica cysts, and serum specimens analyzed for immunoglobulin G (IgG) responses to eight recombinant antigens from G. intestinalis (variant-specific surface protein [VSP1-VSP5]), E. histolytica (lectin adhesion molecule [LecA]), and Cryptosporidium parvum (17- and 27-kDa) using a multiplex bead assay. The IgG responses to VSP antigens peaked at 2 years of age and then diminished and were significantly lower (P < 0.002) in children > 4.5 years than in children < 4.5 years. The IgG responses to Cryptosporidium tended to increase with age. The IgG responses to LecA and VSP antigens and the prevalence of stools positive for cysts were significantly higher (P < 0.037 and P < 0.035, respectively) in the rainy season than in the dry season. The multiplex bead assay provides a powerful tool for analyzing serologic responses to multiple pathogens.

MeSH terms

  • Antibodies, Protozoan / immunology*
  • Child
  • Child, Preschool
  • Cryptosporidiosis / epidemiology
  • Cryptosporidium parvum / immunology*
  • Entamoeba histolytica / immunology*
  • Entamoeba histolytica / isolation & purification
  • Entamoebiasis / epidemiology
  • Feces / parasitology*
  • Giardia lamblia / immunology*
  • Giardia lamblia / isolation & purification
  • Giardiasis / epidemiology
  • Haiti / epidemiology
  • Humans
  • Immunoglobulin G / immunology*
  • Infant
  • Infant, Newborn
  • Longitudinal Studies
  • Parasite Egg Count
  • Protozoan Infections / epidemiology*
  • Serologic Tests

Substances

  • Antibodies, Protozoan
  • Immunoglobulin G