[In vitro derivation and characterization of a colorectal cancer stem cell subpopulation]

A P Davydov-Sinitsyn; O V Bazhenova; M A Liskovykh; L L Chechik; C V Ponomartsev; A N Tomilin; E N Tolkunova

[In vitro derivation and characterization of a colorectal cancer stem cell subpopulation]

Tsitologiia. 2013;55(5):318-23.

[Article in Russian]

Authors

A P Davydov-Sinitsyn, O V Bazhenova, M A Liskovykh, L L Chechik, C V Ponomartsev, A N Tomilin, E N Tolkunova

PMID: 24592739

Abstract

In present publication we describe for the first time the obtainment of cancer stem cells from a weakly metastatic human colorectal carcinoma cell line MIP101 via selecting from the native population the cells that express intensively an embryonic stem cell marker, POU5F1 (Oct4). We provide the evidence that these cells possess an elevated clonogenic and tumorigenic potential when compared to the native population, and this correlates to the hypothesis of cancer stem cells' primary role in the development of malignant neoplasms.

Publication types

English Abstract

MeSH terms

Animals
Biomarkers, Tumor / genetics
Cell Line, Tumor
Cell Lineage / genetics
Colorectal Neoplasms / etiology
Colorectal Neoplasms / genetics*
Colorectal Neoplasms / pathology
Embryonic Stem Cells / cytology*
Embryonic Stem Cells / metabolism
Gene Expression Regulation, Neoplastic
Humans
Mice
Neoplasm Metastasis / genetics
Neoplasm Metastasis / pathology
Neoplastic Stem Cells / cytology*
Neoplastic Stem Cells / metabolism
Octamer Transcription Factor-3 / biosynthesis*

Substances

Biomarkers, Tumor
Octamer Transcription Factor-3
POU5F1 protein, human