Genome-wide re-sequencing of multidrug-resistant Mycobacterium leprae Airaku-3

Clin Microbiol Infect. 2014 Oct;20(10):O619-22. doi: 10.1111/1469-0691.12609. Epub 2014 Mar 29.

Abstract

Genotyping and molecular characterization of drug resistance mechanisms in Mycobacterium leprae enables disease transmission and drug resistance trends to be monitored. In the present study, we performed genome-wide analysis of Airaku-3, a multidrug-resistant strain with an unknown mechanism of resistance to rifampicin. We identified 12 unique non-synonymous single-nucleotide polymorphisms (SNPs) including two in the transporter-encoding ctpC and ctpI genes. In addition, two SNPs were found that improve the resolution of SNP-based genotyping, particularly for Venezuelan and South East Asian strains of M. leprae.

Keywords: Leprosy; Mycobacterium leprae; molecular epidemiology; rifampicin-resistance; single nucleotide polymorphism.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Asia, Southeastern
  • Drug Resistance, Multiple, Bacterial*
  • Genome, Bacterial
  • Genotype
  • Humans
  • Leprosy / microbiology
  • Molecular Sequence Data
  • Mycobacterium leprae / classification
  • Mycobacterium leprae / genetics*
  • Phylogeny
  • Polymorphism, Single Nucleotide
  • Sequence Analysis, DNA / methods*
  • Venezuela

Associated data

  • SRA/SRR113282