Abstract
A murine monoclonal antibody (MAB AF-1) class IgM was raised to a soluble glucomannoprotein extract (GMP) of Candida albicans. Agglutination and indirect immunofluorescence assays with purified MAB showed that AF-1 was directed against a cell-surface epitope shared by C. albicans serotypes A and B, C. tropicalis, C. guilliermondii and C. viswanathii, but not by C. krusei, C. parapsilosis, C. kefyr (pseudotropicalis) and T. glabrata. Treatment with heat, protease or periodate-treated GMP and with other cell-wall extracts of C. albicans provided evidence that the epitope recognised by MAB AF-1 is carried by the polysaccharide moiety of cell-surface glucomannoprotein molecule(s).
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Agglutination Tests
-
Animals
-
Antibodies, Monoclonal / analysis
-
Antibodies, Monoclonal / biosynthesis*
-
Antibodies, Monoclonal / isolation & purification
-
Antibody Specificity
-
Antigens, Fungal / immunology*
-
Antigens, Surface / immunology
-
Candida / immunology*
-
Candida / ultrastructure
-
Candida albicans / immunology*
-
Candida albicans / ultrastructure
-
Cell Wall / immunology
-
Enzyme-Linked Immunosorbent Assay
-
Epitopes / immunology
-
Fluorescent Antibody Technique
-
Glycoproteins / immunology*
-
Humans
-
Hybridomas
-
Immunodiffusion
-
Immunoglobulin M / analysis
-
Immunoglobulin M / biosynthesis
-
Immunoglobulin M / isolation & purification
-
Mannans / immunology
-
Membrane Glycoproteins*
-
Mice
Substances
-
Antibodies, Monoclonal
-
Antigens, Fungal
-
Antigens, Surface
-
Epitopes
-
Glycoproteins
-
Immunoglobulin M
-
Mannans
-
Membrane Glycoproteins
-
mannoproteins