Homologous recombination in human embryonic stem cells using CRISPR/Cas9 nickase and a long DNA donor template

Protein Cell. 2014 Apr;5(4):258-60. doi: 10.1007/s13238-014-0032-5.

Authors

Zhili Rong¹, Shengyun Zhu, Yang Xu, Xuemei Fu

Affiliation

¹ Division of Biological Sciences, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, 92093, USA.

No abstract available

Publication types

Letter
Research Support, Non-U.S. Gov't

MeSH terms

Cell Line
Clustered Regularly Interspaced Short Palindromic Repeats / genetics*
DNA / metabolism
Deoxyribonuclease I / metabolism*
Embryonic Stem Cells / cytology
Embryonic Stem Cells / metabolism*
Homologous Recombination
Humans
Pulmonary Surfactant-Associated Protein C / genetics

Substances

Pulmonary Surfactant-Associated Protein C
DNA
Deoxyribonuclease I