Differential expression and anti-oxidant function of glutathione peroxidase 3 in mouse uterus during decidualization

FEBS Lett. 2014 May 2;588(9):1580-9. doi: 10.1016/j.febslet.2014.02.043. Epub 2014 Mar 11.

Abstract

Glutathione peroxidase 3 (GPX3) is an important member of antioxidant enzymes for reducing reactive oxygen species and maintaining the oxygen balance. Gpx3 mRNA is strongly expressed in decidual cells from days 5 to 8 of pregnancy. After pregnant mice are treated with GPX inhibitor for 3 days, pregnancy rate is significantly reduced. Progesterone stimulates Gpx3 expression through PR/HIF1α in mouse endometrial stromal cells. In the decidua, the high level of GPX3 expression is closely associated with the reduction of hydrogen peroxide (H2O2). Based on our data, GPX3 may play a major role in reducing H2O2 during decidualization.

Keywords: Decidualization; Gpx3; Implantation; Progesterone; Uterus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antioxidants / pharmacology
  • Cell Hypoxia
  • Corpus Luteum / drug effects
  • Decidua / enzymology*
  • Decidua / physiology
  • Embryo Implantation
  • Female
  • Gene Expression Regulation, Enzymologic*
  • Glutathione Peroxidase / antagonists & inhibitors
  • Glutathione Peroxidase / genetics
  • Glutathione Peroxidase / metabolism*
  • Humans
  • Hydrogen Peroxide / metabolism
  • Hypoxia-Inducible Factor 1, alpha Subunit / metabolism
  • Mice
  • Oxidation-Reduction
  • Pregnancy
  • Progesterone / physiology
  • Selenic Acid / pharmacology
  • Thiomalates / pharmacology
  • Transcription, Genetic / drug effects

Substances

  • Antioxidants
  • Hif1a protein, mouse
  • Hypoxia-Inducible Factor 1, alpha Subunit
  • Thiomalates
  • Progesterone
  • 2-thiomalic acid
  • Hydrogen Peroxide
  • Gpx3 protein, mouse
  • Glutathione Peroxidase
  • Selenic Acid