Biosensor based on lectin and lipid membranes for detection of serum glycoproteins in infected patients with dengue

Chem Phys Lipids. 2014 May:180:7-14. doi: 10.1016/j.chemphyslip.2014.02.008. Epub 2014 Mar 11.

Abstract

In this work, we developed a biosystem based on Concanavalin A (ConA) and lipid membranes to recognize glycoproteins from the serum of patients contaminated with dengue serotypes 1, 2 and 3 (DENV1, DENV2 and DENV3). The modified gold electrode was characterized using cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and atomic force microscopy. Morphological analyses of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), DPPC-ConA, DPPC-ConA-DENV1, DPPC-ConA-DENV2 and DPPC-ConA-DENV3 revealed the existence of a non-uniform covering and large globules. EIS and CV measurements have shown that redox probe reactions on the modified gold electrodes were partially blocked due to the adsorption of lipid-ConA system and reveal the interaction response of the immobilized ConA to the presence of glycoproteins of dengue serum. The biosystem exhibited a wide linear response to different concentrations of sera of dengue serotypes 1, 2 and 3. A higher impedimetric response to glycoproteins present in dengue serotype 3 was observed. Our results demonstrate the applicability of lectin and lipid membranes to the development of biosensors for dengue infections.

Keywords: Biosensor; Dengue; Glycoproteins; Lectin; Phospholipid.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 1,2-Dipalmitoylphosphatidylcholine / metabolism*
  • Biosensing Techniques / instrumentation
  • Biosensing Techniques / methods*
  • Cell Membrane*
  • Concanavalin A / metabolism*
  • Dengue / blood*
  • Dengue / diagnosis
  • Electrochemistry
  • Electrodes
  • Glycoproteins / blood*
  • Gold / chemistry
  • Humans
  • Liposomes / metabolism
  • Point-of-Care Systems

Substances

  • Glycoproteins
  • Liposomes
  • Concanavalin A
  • 1,2-Dipalmitoylphosphatidylcholine
  • Gold