Cross-linked iron oxide nanoparticles for therapeutic engineering and in vivo monitoring of mesenchymal stem cells in cerebral ischemia model

Macromol Biosci. 2014 Mar;14(3):380-9. doi: 10.1002/mabi.201300340. Epub 2013 Oct 8.

Abstract

Poly(ethylene glycol)-coated cross-linked iron oxide nanoparticles (PCIONs) are developed for therapeutic engineering of mesenchymal stem cells (MSCs) and their monitoring via magnetic resonance (MR) imaging at a time. PCIONs successfully combine with plasmid DNA (pDNA) via ionic interaction. Accordingly, PCION/pDNA complexes mediate superior translocations of vascular endothelial growth factor (VEGF) pDNA into intracellular regions of MSCs under external magnetic field, which significantly elevate production of VEGF from MSCs. Genetically engineered MSCs are also clearly visualized via MR imaging after administration to rat cerebrovascular ischemia models, which enable tracking of MSCs migration from injected sites to injured ischemic area.

Keywords: cell engineering; cerebral ischemia model; cross-linked iron oxide nanoparticles; magnetic resonance imaging; mesenchymal stem cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain Ischemia / metabolism
  • Brain Ischemia / pathology
  • Brain Ischemia / therapy*
  • Cell Engineering
  • Cell Tracking / methods*
  • Cell- and Tissue-Based Therapy / methods*
  • Disease Models, Animal
  • Ferric Compounds / chemistry
  • Magnetic Resonance Imaging
  • Mesenchymal Stem Cells / cytology
  • Mesenchymal Stem Cells / metabolism*
  • Nanoparticles / chemistry*
  • Plasmids / chemistry
  • Polyethylene Glycols / chemistry
  • Rats
  • Rats, Sprague-Dawley
  • Transfection
  • Vascular Endothelial Growth Factor A / genetics
  • Vascular Endothelial Growth Factor A / metabolism

Substances

  • Ferric Compounds
  • Vascular Endothelial Growth Factor A
  • vascular endothelial growth factor A, rat
  • ferric oxide
  • Polyethylene Glycols