Her2 expression and gene amplification is rarely detectable in patients with oral squamous cell carcinomas

J Oral Pathol Med. 2014 Apr;43(4):304-8. doi: 10.1111/jop.12173. Epub 2014 Mar 20.

Abstract

Purpose: Her2 (ErbB2) transforms cells when overexpressed and is an important therapeutic target in breast cancer. Contrary to breast cancer, studies on Her2 overexpression and gene amplification in squamous cell carcinomas of the head and neck region described largely different results. This study was undertaken to learn more on the prevalence and clinical significance of HER2 amplification and overexpression in squamous cell carcinomas of the head and neck.

Materials and methods: Her2 expression and gene amplification was analyzed by immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH) on two tissue microarrays composed of 427 squamous cell carcinomas of the head and neck region and 222 oral squamous cell carcinomas. Results were compared with clinicopathological features.

Results: Her2 expression and gene amplification was rarely detectable in squamous cell carcinomas of the head and neck region and unrelated to tumor phenotype or survival of the patients with oral squamous carcinoma.

Discussion: Our results demonstrate that Her2 protein and gene amplification was only detectable in a small subset of squamous cell carcinomas of the head and neck region as well as oral squamous cell carcinomas. However, it can be speculated that those few patients with Her2 overexpressing and gene amplificated tumors may possibly benefit from an anti-Her2 therapy.

Keywords: Her2; fluorescence in situ hybridization; head and neck squamous cell carcinoma; immunohistochemistry; oral squamous cell carcinomas.

Publication types

  • Comparative Study

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Carcinoma, Squamous Cell / chemistry*
  • Carcinoma, Squamous Cell / genetics
  • Carcinoma, Squamous Cell / pathology
  • Carcinoma, Squamous Cell / secondary
  • Female
  • Follow-Up Studies
  • Gene Amplification / genetics*
  • Gene Expression Regulation, Neoplastic / genetics
  • Head and Neck Neoplasms / chemistry
  • Head and Neck Neoplasms / genetics
  • Head and Neck Neoplasms / pathology
  • Humans
  • Immunohistochemistry
  • In Situ Hybridization, Fluorescence
  • Lymph Nodes / pathology
  • Male
  • Middle Aged
  • Mouth Floor / chemistry
  • Mouth Floor / pathology
  • Mouth Neoplasms / chemistry*
  • Mouth Neoplasms / genetics
  • Mouth Neoplasms / pathology
  • Neoplasm Grading
  • Neoplasm Recurrence, Local / pathology
  • Neoplasm Staging
  • Pharyngeal Neoplasms / chemistry
  • Pharyngeal Neoplasms / genetics
  • Pharyngeal Neoplasms / pathology
  • Phenotype
  • Receptor, ErbB-2 / analysis*
  • Receptor, ErbB-2 / genetics
  • Survival Rate
  • Tissue Array Analysis / methods

Substances

  • ERBB2 protein, human
  • Receptor, ErbB-2