Detection of changes in the structure and distribution map of triacylglycerol in fatty liver model by MALDI-SpiralTOF

Kahoko Nishikawa; Masahiro Hashimoto; Yoshiyuki Itoh; Sadayuki Hiroi; Akihiko Kusai; Fumihiko Hirata; Toshihisa Sakamoto; Keiichi Iwaya

doi:10.1016/j.fob.2014.02.005

Detection of changes in the structure and distribution map of triacylglycerol in fatty liver model by MALDI-SpiralTOF

FEBS Open Bio. 2014 Feb 19:4:179-84. doi: 10.1016/j.fob.2014.02.005. eCollection 2014.

Authors

Kahoko Nishikawa¹, Masahiro Hashimoto², Yoshiyuki Itoh², Sadayuki Hiroi³, Akihiko Kusai², Fumihiko Hirata⁴, Toshihisa Sakamoto¹, Keiichi Iwaya⁵

Affiliations

¹ Department of Traumatology and Critical Care Medicine, National Defense Medical College, Saitama 359-8513, Japan.
² JEOL Ltd., Akishima, Tokyo 196-8553, Japan.
³ Department of Laboratory Medicine, National Defense Medical College, Saitama 359-8513, Japan.
⁴ Japan Self Defense Forces Hospital Yokosuka, Kanagawa 237-0071, Japan.
⁵ Department of Pathology, National Defense Medical College, Saitama 359-8513, Japan.

Abstract

Matrix-assisted laser desorption/ionisation spiral orbit-type time-of-flight mass spectrometry (MALDI-SpiralTOF) can analyse lipid profiles and characterise lipid structure. Imaging mass spectrometry (IMS) also provides distribution maps of selected m/z values. Here, we investigated triacylglycerol (TG) structure and distribution using these technologies to estimate mouse fatty liver. The distribution and intensity of the most intense mass spectrum ion was indicated by IMS at m/z 881.7 (52:2). Analysis using MS/MS showed a structural change between liver TG and dietary TG. These findings suggest that MALDI-SpiralTOF is a powerful tool for clinical screening and estimating fatty liver.

Keywords: CID, collision-induced dissociation; Fatty liver; HE, haematoxlylin and eosin; HF, high-fat diet; HF + Res, high-fat diet supplemented with 0.2% resveratrol; IMS, imaging mass spectrometry; Imaging mass spectrometry; MALDI-SpiralTOF, matrix-assisted laser desorption/ionisation spiral orbit-type time-of-flight mass spectrometry; Matrix-assisted laser desorption/ionisation; Spiral orbit-type time-of-flight mass spectrometer; TG, triacylglycerol; TOF/TOF, tandem time-of-flight; Triacylglycerol.