Description of a new Mycobacterium intracellulare pattern of PCR restriction enzyme analysis of hsp65 gene

Diagn Microbiol Infect Dis. 2014 Jun;79(2):240-1. doi: 10.1016/j.diagmicrobio.2014.01.028. Epub 2014 Feb 20.

Abstract

This work comprises 9 pulmonary nontuberculous mycobateria isolates obtained from sputum of 4 different patients from Brazil. The sequencing and phylogenetic analysis allowed their accurate identification as Mycobacterium intracellulare. We report a mutation at position 453 creating a new HaeIII cutting site and, therefore, a new PRA-hsp65 M. intracellulare profile.

Keywords: Mycobacterium intracellulare; Nontuberculous mycobacteria; PRA-hsp65.

MeSH terms

  • Bacterial Proteins / genetics*
  • Brazil
  • Chaperonin 60 / genetics*
  • Cluster Analysis
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics
  • Humans
  • Molecular Sequence Data
  • Molecular Typing*
  • Mycobacterium avium Complex / classification*
  • Mycobacterium avium Complex / genetics*
  • Mycobacterium avium Complex / isolation & purification
  • Mycobacterium avium-intracellulare Infection / microbiology
  • Phylogeny
  • Pneumonia / microbiology
  • Polymerase Chain Reaction*
  • RNA, Ribosomal, 16S / genetics
  • Restriction Mapping*
  • Sequence Analysis, DNA
  • Sputum / microbiology

Substances

  • Bacterial Proteins
  • Chaperonin 60
  • DNA, Bacterial
  • DNA, Ribosomal
  • RNA, Ribosomal, 16S
  • heat-shock protein 65, Mycobacterium

Associated data

  • GENBANK/KF030698
  • GENBANK/KF030699
  • GENBANK/KF186673
  • GENBANK/KF186674
  • GENBANK/KF186675
  • GENBANK/KF186676
  • GENBANK/KF186677
  • GENBANK/KF186678