Measuring cone density in a Japanese macaque (Macaca fuscata) model of age-related macular degeneration with commercially available adaptive optics

Adv Exp Med Biol. 2014:801:309-16. doi: 10.1007/978-1-4614-3209-8_39.

Abstract

The aim of this study was to assess the feasibility of using a commercially available high-resolution adaptive optics (AO) camera to image the cone mosaic in Japanese macaques (Macaca fuscata) with dominantly inherited drusen. The macaques examined develop drusen closely resembling those seen in humans with age-related macular degeneration (AMD). For each animal, we acquired and processed images from the AO camera, montaged the results into a composite image, applied custom cone-counting software to detect individual cone photoreceptors, and created a cone density map of the macular region. We conclude that flood-illuminated AO provides a promising method of visualizing the cone mosaic in nonhuman primates. Future studies will quantify the longitudinal change in the cone mosaic and its relationship to the severity of drusen in these animals.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Axial Length, Eye / pathology
  • Cell Count / instrumentation
  • Cell Count / methods
  • Disease Models, Animal*
  • Fundus Oculi*
  • Image Processing, Computer-Assisted / instrumentation
  • Image Processing, Computer-Assisted / methods
  • Macaca*
  • Macular Degeneration / pathology*
  • Neoplasms, Basal Cell
  • Ophthalmoscopy / methods
  • Optic Disk Drusen / pathology*
  • Retinal Cone Photoreceptor Cells / cytology*