Abstract
Pertussis toxin activates T lymphocytes by a mechanism that is independent of its ADP-ribosylation activity. The toxin stimulates increases in diacylglycerol and intracellular calcium apparently by interacting with a cell surface receptor. Consistent with the production of these second messengers we have found that pertussis toxin activates protein kinase C in the Jurkat cell line. The toxin was also found to activate a tyrosine protein kinase in these cells in a manner similar to that observed with phytohemagglutinin. These results provide evidence that the mechanism of activation of T cells by pertussis toxin involves stimulating the activity of protein kinase C and a tyrosine protein kinase.
Publication types
-
Comparative Study
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Calcium / metabolism
-
Cell Line
-
Diglycerides / metabolism
-
Enzyme Activation
-
Humans
-
Immunoblotting
-
Molecular Weight
-
Pertussis Toxin*
-
Phosphorylation
-
Phosphotyrosine
-
Phytohemagglutinins / pharmacology
-
Protein Kinase C / metabolism*
-
Protein-Tyrosine Kinases / metabolism*
-
T-Lymphocytes / enzymology*
-
Tetradecanoylphorbol Acetate / pharmacology
-
Tyrosine / analogs & derivatives
-
Tyrosine / metabolism
-
Virulence Factors, Bordetella / pharmacology*
Substances
-
Diglycerides
-
Phytohemagglutinins
-
Virulence Factors, Bordetella
-
Phosphotyrosine
-
Tyrosine
-
Pertussis Toxin
-
Protein-Tyrosine Kinases
-
Protein Kinase C
-
Tetradecanoylphorbol Acetate
-
Calcium