Plant genome engineering in full bloom

Trends Plant Sci. 2014 May;19(5):284-7. doi: 10.1016/j.tplants.2014.02.014. Epub 2014 Mar 24.

Abstract

The recent development of tools for precise editing of user-specified sequences is rapidly changing the landscape for plant genetics and biotechnology. It is now possible to target mutations and regulatory proteins to specific sites in a genome using zinc-finger nucleases (ZFNs), transcription activator-like endonucleases (TALENs), or the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) system. Here we provide an update of recent developments in CRISPR/Cas9 technology and highlight online resources that will help biologists adopt new genome-editing tools.

Keywords: CRISPR; Cas9; TALENs; ZFNs; genome engineering; plant.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Biotechnology
  • CRISPR-Cas Systems / genetics
  • Clustered Regularly Interspaced Short Palindromic Repeats / genetics*
  • Endonucleases / genetics
  • Gene Expression Regulation, Plant
  • Gene Targeting / methods*
  • Genetic Engineering / methods*
  • Genome, Plant / genetics*
  • Mutation
  • Plants / genetics*
  • Systems Biology*
  • Zinc Fingers

Substances

  • Endonucleases