Molecular characterization and tissue expression of peptide YY in Schizothorax prenanti: effects of periprandial changes and fasting on expression in the hypothalamus

Dengyue Yuan; Chaowei Zhou; Tao Wang; Fangjun Lin; Hu Chen; Hongwei Wu; Rongbin Wei; Zhiming Xin; Ju Liu; Yundi Gao; Defang Chen; Shiyong Yang; Yan Wang; Yundan Pu; Zhiqiong Li

doi:10.1016/j.regpep.2014.03.004

Molecular characterization and tissue expression of peptide YY in Schizothorax prenanti: effects of periprandial changes and fasting on expression in the hypothalamus

Regul Pept. 2014 May:190-191:32-8. doi: 10.1016/j.regpep.2014.03.004. Epub 2014 Mar 26.

Authors

Dengyue Yuan¹, Chaowei Zhou¹, Tao Wang¹, Fangjun Lin¹, Hu Chen¹, Hongwei Wu¹, Rongbin Wei¹, Zhiming Xin¹, Ju Liu¹, Yundi Gao¹, Defang Chen¹, Shiyong Yang¹, Yan Wang¹, Yundan Pu¹, Zhiqiong Li²

Affiliations

¹ Department of Aquaculture, College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, China.
² Department of Aquaculture, College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, China. Electronic address: [email protected].

PMID: 24681121
DOI: 10.1016/j.regpep.2014.03.004

Abstract

Peptide YY (PYY) is a potent anorectic neuropeptide implicated in feeding regulation in mammals. However, the involvement of PYY in the feeding behavior of teleosts has not been well understood. In this study, we employed molecular, real-time quantitative PCR and physiological studies to characterize the structure, distribution, and appetite regulatory effects of PYY in Schizothorax prenanti (S. prenanti). A very high conservation in PYY sequences was found in teleosts. PYY is widely expressed, with the highest levels of expression in telencephalon, medulla oblongata, pituitary and hypothalamus of S. prenanti. The PYY mRNA expression in the hypothalamus was highly elevated after a meal, suggesting a satiety signal role for PYY in S. prenanti. In addition, PYY gene expression in the hypothalamus was decreased after fasting and increased sharply after refeeding, which suggested that PYY might be involved in the central regulation of appetite in S. prenanti. Overall, our result provides basis for further investigation into the regulation of feeding in S. prenanti.

Keywords: Fasting; Molecular characterization; PYY; Postprandial; Schizothorax prenanti.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cloning, Molecular
Cyprinidae / metabolism*
Fasting*
Gene Expression Profiling
Hypothalamus / metabolism*
Peptide YY / genetics*
Peptide YY / metabolism*
Postprandial Period*
Protein Conformation
RNA, Messenger / genetics
RNA, Messenger / metabolism

Substances

RNA, Messenger
Peptide YY