Organic acid quantitation by NeuCode methylamidation

Anal Chem. 2014 May 6;86(9):4402-8. doi: 10.1021/ac500270q. Epub 2014 Apr 11.

Abstract

We have developed a multiplexed quantitative analysis method for carboxylic acids by liquid chromatography high resolution mass spectrometry. The method employs neutron encoded (NeuCode) methylamine labels ((13)C or (15)N enriched) that are affixed to carboxylic acid functional groups to enable duplex quantitation via mass defect measurement. This work presents the first application of NeuCode quantitation to small molecules. We have applied this technique to detect adulteration of olive oil by quantitative analysis of fatty acid methyl amide derivatives, and the quantitative accuracy of the NeuCode analysis was validated by GC/MS. Currently, the method enables duplex quantitation and is expandable to at least 6-plex analysis.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Acids / analysis*
  • Amides / chemistry*
  • Gas Chromatography-Mass Spectrometry
  • Organic Chemicals / analysis*

Substances

  • Acids
  • Amides
  • Organic Chemicals