Inhibition of HIV replication in cell culture by the specific aspartic protease inhibitor pepstatin A

FEBS Lett. 1989 Apr 24;247(2):349-52. doi: 10.1016/0014-5793(89)81368-7.

Abstract

After incubation of H9 cells infected with human immunodeficiency virus (HIV) with pepstatin A at 10(-4) M for 2, 4, or 11 days, the culture medium contained significantly less HIV core antigen (p24) than controls without pepstatin A and no or only borderline activity of reverse transcriptase was detected. In addition, after pepstatin A treatment no infectious HIV at 2 or 4 days and only minimal amounts at 11 days were detectable in the culture medium.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aspartic Acid Endopeptidases
  • Cell Line
  • Endopeptidases
  • HIV / immunology
  • HIV / physiology*
  • HIV Antigens / biosynthesis
  • HIV Core Protein p24
  • Oligopeptides / pharmacology*
  • Pepstatins / pharmacology*
  • Protease Inhibitors*
  • RNA-Directed DNA Polymerase / metabolism
  • Retroviridae Proteins / metabolism
  • Virus Replication / drug effects*

Substances

  • HIV Antigens
  • HIV Core Protein p24
  • Oligopeptides
  • Pepstatins
  • Protease Inhibitors
  • Retroviridae Proteins
  • Streptomyces pepsin inhibitor
  • RNA-Directed DNA Polymerase
  • Endopeptidases
  • Aspartic Acid Endopeptidases
  • pepstatin