Abstract
We have prepared an mAb specific for a human cell surface component (termed anti-Fas mAb). Anti-Fas shows cell-killing activity that is indistinguishable from the cytolytic activity of TNF. Fas antigen was characterized by western blotting, indicating that Fas antigen is a cell surface protein with a molecular weight of 200,000, which is different from the molecular weight of TNF-R. Fas antigen, however, is co-downregulated with the TNF-R when cells sensitive to the cytolytic activity of TNF are incubated with either TNF or anti-Fas. In contrast, Fas antigen on cells insensitive to TNF is not co-downregulated with the TNF-R. We suggest that the cell-killing activity of TNF is mediated by Fas antigen associated with the TNF-R.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Monoclonal / immunology*
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Antigens, Surface / analysis
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Antigens, Surface / immunology*
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Blotting, Western
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Cell Survival
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Colonic Neoplasms
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Drug Synergism
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Electrophoresis, Polyacrylamide Gel
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Female
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Humans
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Immunoglobulin M
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Interferon-gamma / pharmacology
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Mice
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Mice, Inbred BALB C
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Molecular Weight
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Receptors, Cell Surface / physiology*
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Receptors, Tumor Necrosis Factor
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Rhabdomyosarcoma
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Tumor Cells, Cultured
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Tumor Necrosis Factor-alpha / pharmacology
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fas Receptor
Substances
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Antibodies, Monoclonal
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Antigens, Surface
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Immunoglobulin M
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Receptors, Cell Surface
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Receptors, Tumor Necrosis Factor
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Tumor Necrosis Factor-alpha
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fas Receptor
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Interferon-gamma