Diagnosis of early pancreas graft failure via antibody-mediated rejection: single-center experience with 256 pancreas transplantations

Am J Transplant. 2014 Apr;14(4):936-42. doi: 10.1111/ajt.12624. Epub 2014 Feb 12.

Abstract

Early pancreas graft loss is usually attributed to technical failure while the possibility of antibody-mediated rejection (AMR) is generally overlooked. To investigate the role of AMR in early pancreas graft loss, we retrospectively assessed 256 patients with simultaneous pancreas-kidney transplantation (SPK) between 1985 and 2010 at our institute. We included 33 SPK patients who lost their pancreas graft <1 year after transplantation. AMR was diagnosed based on donor-specific antibodies, C4d and histology in 7 cases, 8 cases were suspicious for AMR and 18 pancreas graft losses were not due to AMR. Acute AMR occurred >1 month after transplantation in 6/7 cases, whereas all other causes typically led to loss <1 month after transplantation. Thrombotic lesions occurred equally among the 33 cases. In 12/18 concurrent kidney specimens, the diagnostic results paralleled those of the pancreas graft. All patients with acute AMR of the pancreas graft lost their renal grafts <1 year after transplantation. In the setting of a thrombotic event, histopathological analysis of early pancreas graft loss is advisable to rule out the possibility of AMR, particularly because a diagnosis of acute AMR has important consequences for renal graft outcomes.

Keywords: Antibodies; antibody-mediated rejection; complement C4d; graft rejection; histopathology; pancreas transplantation.

Publication types

  • Clinical Trial
  • Comparative Study

MeSH terms

  • Adult
  • Allografts
  • Case-Control Studies
  • Complement C4b / immunology
  • Female
  • Follow-Up Studies
  • Graft Rejection / diagnosis*
  • Graft Rejection / etiology
  • Graft Rejection / mortality
  • Humans
  • Immunity, Cellular / immunology
  • Isoantibodies / blood*
  • Isoantibodies / immunology
  • Kidney Transplantation / adverse effects*
  • Male
  • Middle Aged
  • Pancreas Transplantation / adverse effects*
  • Pancreatic Diseases / complications*
  • Pancreatic Diseases / surgery
  • Peptide Fragments / immunology
  • Postoperative Complications / diagnosis*
  • Postoperative Complications / etiology
  • Postoperative Complications / mortality
  • Prognosis
  • Retrospective Studies
  • Risk Factors
  • Survival Rate
  • Thrombosis / physiopathology*
  • Tissue Donors

Substances

  • Isoantibodies
  • Peptide Fragments
  • Complement C4b
  • complement C4d