A two-armed lanthanoid-chelating paramagnetic NMR probe linked to proteins via thioether linkages

Wei-Min Liu; Simon P Skinner; Monika Timmer; Anneloes Blok; Mathias A S Hass; Dmitri V Filippov; Mark Overhand; Marcellus Ubbink

doi:10.1002/chem.201400257

A two-armed lanthanoid-chelating paramagnetic NMR probe linked to proteins via thioether linkages

Chemistry. 2014 May 19;20(21):6256-8. doi: 10.1002/chem.201400257. Epub 2014 Apr 15.

Authors

Wei-Min Liu¹, Simon P Skinner, Monika Timmer, Anneloes Blok, Mathias A S Hass, Dmitri V Filippov, Mark Overhand, Marcellus Ubbink

Affiliation

¹ Leiden Institute of Chemistry, Leiden University, Post Office Box 9502, 2300 RA Leiden (The Netherlands).

PMID: 24737492
DOI: 10.1002/chem.201400257

Abstract

Paramagnetic NMR probes provide valuable long-range structural information on proteins and protein complexes. A new, stable, two-armed lanthanoid probe is reported that can be attached to a protein site-specifically via chemically inert thioether linkages.

Keywords: lanthanides; paramagnetic NMR; protein modification; protein structures; pseudocontact shift.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chelating Agents / chemistry*
Lanthanoid Series Elements / chemistry*
Models, Molecular
Molecular Structure
Nuclear Magnetic Resonance, Biomolecular

Substances

Chelating Agents
Lanthanoid Series Elements