Somatic mutation in acute myelogenous leukemia cells imitate novel germline HLA-A allele: a case report

Tissue Antigens. 2014 Jun;83(6):414-7. doi: 10.1111/tan.12362. Epub 2014 Apr 23.

Abstract

A somatic mutation of the human leukocyte antigen (HLA)-A gene revealed in tumour cells of acute myelogenous leukemia (AML) is described. A patient with AML and her siblings were routinely typed for HLA in order to find a suitable donor for haematopoietic stem cell transplantation. Sequencing-based typing of the initial patient's sample characterized by high proportion of blasts revealed unknown G/A exchange at position 781 of the HLA-A gene (exon 4) associated with HLA-A*02:01 allele. Importantly, this G781A variant was completely absent in the patient's remission sample obtained after the clearance of blasts. Our results are a reminder that HLA mutations in tumour cells may interfere with routine HLA typing and should always be considered, namely, in patients with haematological malignancies.

Keywords: human leukocyte antigen typing; major histocompatibility complex; new HLA allele; polymorphism; somatic mutation.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Alleles
  • Amino Acid Substitution
  • Base Sequence
  • Exons / genetics
  • Female
  • Germ-Line Mutation
  • HLA-A2 Antigen / genetics*
  • Hematopoietic Stem Cell Transplantation
  • Histocompatibility Testing
  • Humans
  • Leukemia, Myeloid, Acute / genetics*
  • Leukemia, Myeloid, Acute / pathology
  • Leukemia, Myeloid, Acute / therapy
  • Molecular Sequence Data
  • Mutation, Missense*
  • Neoplastic Stem Cells / chemistry*
  • Point Mutation*
  • Remission Induction
  • Siblings

Substances

  • HLA-A*02:01 antigen
  • HLA-A2 Antigen