Pre-selecting resistance against individual Bti Cry toxins facilitates the development of resistance to the Bti toxins cocktail

Renaud Stalinski; Guillaume Tetreau; Thierry Gaude; Laurence Després

doi:10.1016/j.jip.2014.04.002

Pre-selecting resistance against individual Bti Cry toxins facilitates the development of resistance to the Bti toxins cocktail

J Invertebr Pathol. 2014 Jun:119:50-3. doi: 10.1016/j.jip.2014.04.002. Epub 2014 Apr 21.

Authors

Renaud Stalinski¹, Guillaume Tetreau², Thierry Gaude³, Laurence Després⁴

Affiliations

¹ Laboratoire d'Ecologie Alpine, CNRS-UMR 5553, Université Joseph Fourier, BP 53, 38041 Grenoble cedex 09, France. Electronic address: [email protected].
² Laboratoire d'Ecologie Alpine, CNRS-UMR 5553, Université Joseph Fourier, BP 53, 38041 Grenoble cedex 09, France; Department of Entomology, Cornell University, New York State Agricultural Experiment Station, Geneva, NY 14456, USA. Electronic address: [email protected].
³ Laboratoire d'Ecologie Alpine, CNRS-UMR 5553, Université Joseph Fourier, BP 53, 38041 Grenoble cedex 09, France. Electronic address: [email protected].
⁴ Laboratoire d'Ecologie Alpine, CNRS-UMR 5553, Université Joseph Fourier, BP 53, 38041 Grenoble cedex 09, France. Electronic address: [email protected].

PMID: 24768915
DOI: 10.1016/j.jip.2014.04.002

Abstract

The bioinsecticide Bacillus thuringiensis subsp. israelensis is a larvicide used worldwide for mosquito control, which contains three Cry toxins and one Cyt toxin. We investigated for the first time in Aedes aegypti (1) the evolution of resistance and cross-resistance of strains selected with each Cry toxin, and (2) the effect of pre-selection with Cry toxin on the evolution of resistance to a mix of Bti toxins. Cross resistance was higher between Cry4Ba and Cry11Aa than between Cry4Aa and either Cry4Ba or Cry11Aa, suggesting both common and specific mechanisms of resistance. Pre-selecting resistance to each Cry toxins facilitated the development of resistance to the full Bti toxins cocktail.

Keywords: Aedes aegypti; Bacillus thuringiensis subsp. israelensis; Bioassay; Resistance.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aedes / microbiology*
Animals
Bacillus thuringiensis Toxins
Bacterial Proteins / toxicity*
Endotoxins / toxicity*
Hemolysin Proteins / toxicity*
Insecticide Resistance / genetics*
Mosquito Control / methods*
Pest Control, Biological / methods*

Substances

Bacillus thuringiensis Toxins
Bacterial Proteins
Endotoxins
Hemolysin Proteins
insecticidal crystal protein, Bacillus Thuringiensis