Comparison of the results of PCR analysis of gene expression in breast cancer tissue specimens stabilized in formalin and RNAlater

N A Khaustova; D S Makeeva; O V Kondrashina; N N Fedotov; I N Nechaev; N A Savelov; V N Grinevich; D V Maltseva; V V Galatenko; M Yu Shkurnikov

doi:10.1007/s10517-014-2381-8

Comparison of the results of PCR analysis of gene expression in breast cancer tissue specimens stabilized in formalin and RNAlater

Bull Exp Biol Med. 2014 Feb;156(4):486-90. doi: 10.1007/s10517-014-2381-8. Epub 2014 Mar 6.

Authors

N A Khaustova¹, D S Makeeva, O V Kondrashina, N N Fedotov, I N Nechaev, N A Savelov, V N Grinevich, D V Maltseva, V V Galatenko, M Yu Shkurnikov

Affiliation

¹ BioClinicum Center, Moscow, Russia, [email protected].

PMID: 24771434
DOI: 10.1007/s10517-014-2381-8

Abstract

We compared the results of real-time PCR analysis of gene expression in paired specimens breast cancer tissue fixed in RNAlater (Qiagen) stabilization reagent (FF samples) and in formalin (FFPE samples). A clear-cut linear relationship (correlation coefficient 0.76 ± 0.07) was detected between the gene expression logarithm values in FF and FFPE samples. This fact suggests that collections of paraffin blocks with formalin-fixed tissue specimens from patients with a many-year disease history can be effectively used in modern studies.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Breast Neoplasms / genetics
Breast Neoplasms / metabolism*
Breast Neoplasms / pathology
Carcinoma, Ductal, Breast / genetics
Carcinoma, Ductal, Breast / metabolism*
Carcinoma, Ductal, Breast / pathology
Female
Fixatives / chemistry
Formaldehyde / chemistry
Gene Expression
Gene Expression Profiling / methods*
Humans
Middle Aged
RNA Stability
Reagent Kits, Diagnostic
Tissue Fixation / methods*

Substances

Fixatives
Reagent Kits, Diagnostic
Formaldehyde