The effect of component-resolved diagnosis on specific immunotherapy prescription in children with hay fever

J Allergy Clin Immunol. 2014 Jul;134(1):75-81. doi: 10.1016/j.jaci.2014.01.042. Epub 2014 May 1.

Abstract

Background: Sensitization to profilins and other cross-reacting molecules might hinder proper specific immunotherapy (SIT) prescription in polysensitized patients with pollen-related allergic rhinitis (AR). In these patients, component-resolved diagnosis (CRD) might modify SIT prescription by improving the identification of the disease-eliciting pollen sources.

Objectives: We sought to measure the effect of CRD on SIT prescription in children with pollen-related AR.

Methods: Children (n = 651) with moderate-to-severe pollen-related AR were recruited between May 2009 and June 2011 in 16 Italian outpatient clinics. Skin prick test (SPT) reactivity to grass, cypress, olive, mugwort, pellitory, and/or Betulaceae pollen was considered clinically relevant if symptoms occurred during the corresponding peak pollen season. IgE sensitization to Phl p 1, Phl p 5, Bet v 1, Cup a 1, Art v 1, Ole e 1, Par j 2, and Phl p 12 (profilin) was measured by using ImmunoCAP. SIT prescription was modeled on SPT responses first and then remodeled considering also CRD according to GA(2)LEN-European Academy of Allergology and Clinical Immunology guidelines and the opinions of 14 pediatric allergists.

Results: No IgE to the respective major allergens was detected in significant proportions of patients with supposed clinically relevant sensitization to mugwort (45/65 [69%]), Betulaceae (146/252 [60%]), pellitory (78/257 [30%]), olive (111/390 [28%]), cypress (28/184 [15%]), and grass (56/568 [10%]). IgE to profilins, polcalcins, or both could justify 173 (37%) of 464 of these SPT reactions. After CRD, the SPT-based decision on SIT prescription or composition was changed in 277 (42%) of 651 or 315 (48%) of 651 children according to the European or American approach, respectively, and in 305 (47%) of 651 children according to the opinion of the 14 local pediatric allergists.

Conclusions: In children with pollen-related AR, applying CRD leads to changes in a large proportion of SIT prescriptions as opposed to relying on clinical history and SPT alone. The hypothesis that CRD-guided prescription improves SIT efficacy deserves to be tested.

Keywords: Allergic rhinitis; IgE; children; component-resolved diagnosis; panallergens; pollen; profilin; specific immunotherapy.

Publication types

  • Multicenter Study

MeSH terms

  • Adolescent
  • Allergens / chemistry
  • Allergens / immunology*
  • Child
  • Child, Preschool
  • Cross Reactions
  • Desensitization, Immunologic / methods*
  • Female
  • Gene Expression
  • Humans
  • Immunoglobulin E / blood
  • Male
  • Plants / immunology
  • Pollen / chemistry
  • Pollen / immunology*
  • Profilins / genetics
  • Profilins / immunology
  • Rhinitis, Allergic, Seasonal / diagnosis*
  • Rhinitis, Allergic, Seasonal / immunology
  • Rhinitis, Allergic, Seasonal / pathology
  • Rhinitis, Allergic, Seasonal / therapy*
  • Skin Tests

Substances

  • Allergens
  • Profilins
  • Immunoglobulin E