Modulator effects of meloxicam against doxorubicin-induced nephrotoxicity in mice

J Biochem Mol Toxicol. 2014 Aug;28(8):337-46. doi: 10.1002/jbt.21570. Epub 2014 May 3.

Abstract

Doxorubicin-induced renal toxicity overshadows its anticancer effectiveness. This study is aimed at assessing the possible modulator effects of meloxicam, a cyclooxigenase-2 inhibitor, on doxorubicin-induced nephrotoxicity in mice and exploring some of the modulator mechanisms. Forty male mice were divided for treatment, for 2 weeks, with saline, meloxicam (daily), doxorubicin (twice/week), or both meloxicam and doxorubicin. Doxorubicin induced a significant increase in relative kidney weight to body weight, kidney lipid perooxidation, plasma levels of interleukin-6 and tumor necrosis factor-α, kidney caspase-3 activity, and kidney prostaglandin E2 (PGE2) content. Doxorubicin disturbed kidney histology, abrogated renal function tests (serum creatinine, uric acid, and blood urea nitrogen), induced a significant decrease in antioxidant enzyme activities (superoxide dismutase, glutathione peroxidase, and catalase) and reduced glutathione (GSH) content. The administration of meloxicam with doxorubicin mitigated all doxorubicin-disturbed parameters. Meloxicam ameliorated doxorubicin-induced renal injury via inhibition of inflammatory PGE2, inflammatory cytokines, caspase-3 activity, antioxidant effect, and free radical scavenging activity.

Keywords: Cyclooxygenase-2; Cyclooxygenase-2 Inhibitors; Doxorubicin; Meloxicam.

MeSH terms

  • Animals
  • Caspase 3 / blood
  • Dinoprostone / blood
  • Doxorubicin / administration & dosage
  • Doxorubicin / adverse effects*
  • Humans
  • Interleukin-6 / blood
  • Lipid Peroxidation / drug effects
  • Male
  • Meloxicam
  • Mice
  • Neoplasms / blood
  • Neoplasms / drug therapy
  • Oxidative Stress / drug effects*
  • Renal Insufficiency / chemically induced
  • Renal Insufficiency / drug therapy*
  • Renal Insufficiency / pathology
  • Thiazines / administration & dosage*
  • Thiazoles / administration & dosage*
  • Tumor Necrosis Factor-alpha / blood

Substances

  • Interleukin-6
  • Thiazines
  • Thiazoles
  • Tumor Necrosis Factor-alpha
  • Doxorubicin
  • Caspase 3
  • Dinoprostone
  • Meloxicam